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Yingfu Li, PhD
Associate Professor and Canada Research Chair in
Directed Evolution of
Nucleic Acids
Department of Biochemistry and Biomedical Sciences and Department of Chemistry
McMaster University |
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- Research
- People
- Publications
- Li Lab
Shen Y, Chiuman W, Brennan JD, Li Y. Catalysis and rational engineering of trans-acting pH6DZ1, an RNA-cleaving and fluorescence-signaling deoxyribozyme with a four-way junction structure. Chembiochem. 2006 Sep;7(9):1343-8.
Elowe NH, Nutiu R, Allali-Hassani A, Cechetto JD, Hughes DW, Li Y, Brown ED. Small-molecule screening made simple for a difficult target with a signaling nucleic acid aptamer that reports on deaminase activity. Angew Chem Int Ed Engl. 2006 Aug 25;45(34):5648-52.
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“If cancer stem cells lie at the heart of some cancers, then being able to predict the behavior of tumors and providing effective therapies against them means understanding the abnormal growth pathways within the stem cells themselves.”
- John Dick, Project Leader (UHN)
“The only reason that I'm willing to take a risk on this project is my confidence in the quality and camaraderie of my colleagues.”
- Scott Tanner, Principal Investigator (University of Toronto)
“We're examining DNA not for its well-recognized role as the genetic material to store and transmit genetic information, but rather for its less-known potential to act as a novel cancer diagnostic tool.”
- Yingfu Li, Principal Investigator (McMaster University)
“We're gaining more understanding of cell defects and how to modify them, so that treatment is no longer a shotgun approach, but more like an arrow aimed at a specific target.”
- Mark Minden, Principal Investigator (OCI)
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Shen Y, Chiuman W, Brennan JD, Li Y. Catalysis and rational engineering of trans-acting pH6DZ1, an RNA-cleaving and fluorescence-signaling deoxyribozyme with a four-way junction structure. Chembiochem. 2006 Sep;7(9):1343-8.
Elowe NH, Nutiu R, Allali-Hassani A, Cechetto JD, Hughes DW, Li Y, Brown ED. Small-molecule screening made simple for a difficult target with a signaling nucleic acid aptamer that reports on deaminase activity. Angew Chem Int Ed Engl. 2006 Aug 25;45(34):5648-52.
Navani NK, Li Y. Nucleic acid aptamers and enzymes as sensors. Curr Opin Chem Biol. 2006 Jun;10(3):272-81.
Rupcich N, Nutiu R, Li Y, Brennan JD. Solid-phase enzyme activity assay utilizing an entrapped fluorescence-signaling DNA aptamer. Angew Chem Int Ed Engl. 2006 May 12;45(20):3295-9.
Shen Y, Brennan JD, Li Y. Characterizing the secondary structure and identifying functionally essential nucleotides of pH6DZ1, a fluorescence-signaling and RNA-cleaving deoxyribozyme, Biochemistry 2005 Sep; 44(36):12066-76.
Nutiu R, Li Y. A DNA-Protein Nanoengine for "On-Demand" Release and Precise Delivery of Molecules, Angew. Chem. Int. Ed. 2005 Aug; 44(34):5464-7.
Achenbach JC, Nutiu R, Li, Y. Structure-switching allosteric deoxyribozymes, Analytica Chimica Acta. 2005 Apr 4; 534(1):41-51.
Nutiu R, Li Y. In vitro selection of structure-switching signaling aptamers, Angew Chem Int Ed Engl. 2005 Feb 4;44(7):1061-5.
Nutiu R, Yu JM, Li Y. Signaling aptamers for monitoring enzymatic activity and for inhibitor screening, Chembiochem. 2004 Aug 6;5(8):1139-44.
Nutiu R, Li Y. Structure-switching signaling aptamers: transducing molecular recognition into fluorescence signaling, Chemistry. 2004 Apr 19;10(8):1868-76.
Nutiu R, Li Y. Structure-switching signaling aptamers, J Am Chem Soc. 2003 Apr 23;125(16):4771-8.
Berezovski M, Nutiu R, Li Y, Krylov SN. Affinity analysis of a protein-aptamer complex using nonequilibrium capillary electrophoresis of equilibrium mixtures, Anal Chem. 2003 Mar 15;75(6):1382-6.
Achenbach JC, Jeffries GA, McManus SA, Billen LP, Li Y. Secondary-structure characterization of two proficient kinase deoxyribozymes, Biochemistry. 2005 Mar 15;44(10):3765-74.
Achenbach JC, Chiuman W, Cruz RP, Li Y. DNAzymes: from creation in vitro to application in vivo, Curr Pharm Biotechnol. 2004 Aug;5(4):321-36.
Schlosser K, Li Y. Tracing sequence diversity change of RNA-cleaving deoxyribozymes under increasing selection pressure during in vitro selection, Biochemistry. 2004 Aug 3;43(30):9695-707.
Cruz RP, Withers JB, Li Y. Dinucleotide junction cleavage versatility of 8-17 deoxyribozyme, Chem Biol. 2004 Jan;11(1):57-67.
Liu Z, Mei SH, Brennan JD, Li Y. Assemblage of signaling DNA enzymes with intriguing metal-ion specificities and pH dependences, J Am Chem Soc. 2003 Jun 25;125(25):7539-45.
Mei SH, Liu Z, Brennan JD, Li Y. An efficient RNA-cleaving DNA enzyme that synchronizes catalysis with fluorescence signaling, J Am Chem Soc. 2003 Jan 15;125(2):412-20.
Wang W, Billen LP, Li Y. Sequence diversity, metal specificity, and catalytic proficiency of metal-dependent phosphorylating DNA enzymes, Chem Biol. 2002 Apr;9(4):507-17.
Li Y, Liu Y, Breaker RR. Capping DNA with DNA, Biochemistry. 2000 Mar 21;39(11):3106-14.
Li Y, Breaker RR. Deoxyribozymes: new players in the ancient game of biocatalysis, Curr Opin Struct Biol. 1999 Jun;9(3):315-23.
Li Y, Breaker RR. Phosphorylating DNA with DNA, Proc Natl Acad Sci U S A. 1999 Mar 16;96(6):2746-51.
Li Y, Sen D. A catalytic DNA for porphyrin metallation, Nat Struct Biol. 1996 Sep;3(9):743-7.
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